· Anatomy is the study of internal structure with naked eyes. · Father of plant anatomy is N. Grew. · Nageli is regarded as the father of modern plant anatomy. · Safranin and hematoxylin: Safranin stains lignified elements deep red. Eg; sclerenchyma; and hematoxylin stains cellulose elements purple. Eg; living cells like parenchyma. · Maceration: separation of tissue into its component cells. · Conc HNO3 + KClO3 (Crystal) is used to loosen the middle lamella between the cells. During the preparation of the permanent slides, sections are kept in different grades of alcohol for dehydration. A. Tissue: · Term was coined by N. grew · Defined as a group of similar or dissimilar cells having a common origin and performing a common function. · Anatomically plant is made up of various types of tissue with specific functions. · Depending upon the nature of tissue, plant tissue is divided into 1. Meristematic tissue 2. Permanent tissue 3. Special tissue ...